Honest answer: I’m not sure if there is specific research comparing post-menopausal women and women still experiencing regular menses with regard to benefits of increased daily protein intake. There may be. I’m just not expert on the matter.
There is evidence that men over 50 may benefit from 10-20% more protein intake, daily, than younger counterparts.
Ah, I see. If you meant that you’d take protein shakes during the 12-hr ride, I’d advise against. If you meant that you’d increase your daily protein intake due to the 12-hr ride’s propensity to cause muscle breakdown, I’d still advise against. The focus should be to hit the same daily protein total as all other days, and to maximize carb intake throughout the day of the ride, and especially during the ride.
For context: I do tend to push pretty high protein intake. I’m not shy of protein at all. There are times where I’ll recommend as high as 1.2 g protein per pound of body weight. (that’s a lot, by most folks standards). Just alerting you to this so that you can take that into account when I say that I would not recommend protein intake during a 12-hr event.
The truth is, if you’re not absolutely killing yourself for maximum performance, you may be able to get away with some protein intake. Use whey isolate or hydrolysate. But if optimizing hydration, and fuel intake, for maximum performance, is the goal, then I would stay away from even the most easily digested whey protein during a 12-hr event. If you can stomach more kcal, it should come from more carbs.
Protein consumption is usually ideal around the clock. In the case of a 12-hr ride scenario since it’s suboptimal to have to digest protein during the 12-hr ride, consuming some in the 90-150 minutes before the ride is an optimal way to meet the daily needs. Whether you do consume any pre-workout protein, or not, probably will have little bearing on your performance on during the big ride. That pre-workout protein recommendation stemmed from general health and body composition management advice, rather than performance maximization for the day of a big ride.
These are good questions! And, please do argue! I’m not easily offended and love to have my ideas thoughtfully challenged.