I’d be really surprised if you could feel a difference in the larger jumps. There are only 2 shifts along the cassette that will be any different anyway; all others will retain the exact same gap.
In general, I don’t always understand the desire to retain such small gear jumps at the expense of losing total gear range. Outside of flat time trials, I’ve never wanted it. In fact, in crits and some XC courses, I often find myself preferring bigger jumps. These are basically a bunch of corners and reaccelerations. When I have a tighter spaced cassette block, I find myself spinning up the gear, then grabbing two shifts before hitting it again. I prefer a 32t cassette for crits because sometimes this leads to a bigger jump and I only need to upshift one gear.
Sorry for the rant - just speaking out against a common ‘complaint.’
In related news - if your bike currently has a GRX or Ultegra RX cassette, you can actually fit a 40t cassette, even though Shimano claims only 34t would work. Swapping from the 40t on the wheel over to 32t on the trainer shouldn’t be a big deal. In theory, the b screw adjustment would be too tight on the 32t (assuming it was set properly for the 40t), but I doubt you’d notice any issue with indoor riding.