I use a separate wheel on the trainer than outside. As a matter of fact, I have 3 rear wheels, trainer, road and gravel. There seems to be consistent sound across all wheels. The trainer and road wheels have an 11-32 cassette and the gravel wheel has an 11-34. Ive looked at the B screw or “final adjustment” screw and it seems to be ok across all wheels.
Yes, Im working with 3 different hubs which could all be space slightly differently in regards to the read der.